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Friday, July 20, 2012

BM 2205 BIOCHEMISTRY SYLLABUS | ANNA UNIVERSITY BE BIOMEDICAL ENGINEERING 3RD SEMESTER SYLLABUS REGULATION 2008 2011-2012

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BM 2205 BIOCHEMISTRY SYLLABUS | ANNA UNIVERSITY BE BIOMEDICAL ENGINEERING 3RD SEMESTER SYLLABUS REGULATION 2008 2011-2012 BELOW IS THE ANNA UNIVERSITY THIRD SEMESTER BE BIOMEDICAL ENGINEERING DEPARTMENT SYLLABUS IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011-2012 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI,TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009


BM 2205 BIOCHEMISTRY L T P C
3 0 0 3 AIM
 To study the biochemical reactions and the various methods to analyze them.
OBJECTIVE:
 To give a clear understanding of important biomolecules and their functions.
 To analyze the metabolic pathways in normal and diseased state.
 To help in devising analytical & diagnostic tools.
UNIT I 6
Introduction to biochemistry – Biomolecules, structure of water & its importance –
Important noncovalent forces – Hydrogen bonds, electrostatic, hydrophobic & vanderwaals
forces – Acid, base & buffers – pH, Henderson Hasselbalch equation. Biological buffers
and their significance – Principle of viscosity – surface tension , adsorption, diffusion,
osmosis & their applications in biological systems.
UNIT II 9
Classification, structure & properties of carbohydrates – mono, di , oligo &
polysaccharides. Classification, structure & properties of amino acids & proteins.
Classification, structure & properties of Lipids – Simple lipids , Phospholipids , glycolipids
& steroids . Transport of lipids: Lipoproteins Structure & functions of nucleic acids –
Nucleosides , nucleotides – Cyclic AMP , cyclic GMP , ATP , GTP – DNA & RNA
UNIT III 12
Classification of Enzymes, Chemical nature, Active Site, Specificity of Enzyme catalyzed
reactions, Regulation : Feedback , Allosteric , Covalent modification , Hormonal regulation,
co-enzymes. Assay of enzymes, enzymes in clinical diagnosis of diseases. Introduction to
Metabolism: Carbohydrate metabolism, Glycolysis
30
Lipid metabolism : fatty acid, beta oxidation , ketogenesis and cholesterol metabolism.
TCA cycle : Structure of biological membranes, electron transport & Oxidative
phosphorylation.
UNIT IV 9
Liver function and liver function tests, Kidney function and kidney function tests , normal &
abnormal constituents of urine and their clinical significance. General characteristics of
hormones. Structure , functions & disorders of thyroid , parathyroid , pituitary ,adrenal and
pancreatic hormones. Hormones as chemical messengers: General assay of hormones –
Bio assay , chemical assay & immuno assays.
UNIT V 9
Analytical techniques: Principle and applications of electrophoresis – PAGE , SDS PAGE ,
Isoelectric focusing , Two Dimensional Electrophoresis. Chromatography: Principle of
adsorption & partition chromatography, Size exclusion , Ion exchange & affinity
chromatography. Spectro photometry, fluorimetry, flame photometry, manometry ,
microcalorimetry , electrochemical methods, biosensors , automation in clinical laboratory ,
use of radio isotopes in biochemistry.
TOTAL : 45 PERIODS
TEXT BOOKS: 1. Harper’s review of biochemistry By David. W. Martin, Peter. A. Mayes , Victor. W.
Rodwell . LANGE medical publications, 2003.
2. Practical Biochemistry – Principles & Techniques,Keith Wilson & John Walker. Oxford
university press.
REFERENCE S:
1. Understanding Enzymes By Trevor palmer. Published by Ellis Horwood LTD.
2. Biochemistry Lippincott’s Illustrated Reviews By Pamela.C.Champe & Richard.
A.Harvey. Lippincott-Raven publishers, 3rd edition, 2006.

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