BT2207 BIOCHEMISTRY LABORATORY SYLLABUS | ANNA UNIVERSITY B.TECH. BIOTECHNOLOGY 3RD SEMESTER SYLLABUS REGULATION 2008 2011-2012 BELOW IS THE ANNA UNIVERSITY THIRD SEMESTER B.TECH. BIOTECHNOLOGY DEPARTMENT SYLLABUS IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011-2012 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI,TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT2207 BIOCHEMISTRY LABORATORY L T P C
0 0 4 2
MAIN TOPICS OF STUDY
Demonstration of use of volume and weight measurements devices.
Titration of weak acid-weak base.
Quantitative Test for carbohydrates
Distinguish reducing and nontraducing sugars.
Using ninhydrin for distinguishing Imino and amino acids
Protein estimation by Biuret and Lowry’s methods.
Protein estimation by Bradfrd colorometric methods.
Extraction of lipids and analysis by TLC.
Estimation of nucleus ends by absorbance at 260mm and hyper chromicity.
Enzymatic assay of phosphates.
Hydrolysis of starch by an enzyme
TOTAL: 60 PERIODS
REFERENCES
1. Wilson and Walker “Principles and Techniques of Practical Biochemistry” 4 Edn.,
Cambridge Knew pros 1997.
2. Plummer DT “An Introduction to Practical Biochemistry” III Edn., Tata McGrawhill.
LIST OF EQUIPMENTS
Heating Mantles (5) / Water Baths (5) / Bunsen Buners (10)
TLC Plates – Required Numbers
Colorimeter – 2 Nos.
Consumables and Reagents.
BT2207 BIOCHEMISTRY LABORATORY L T P C
0 0 4 2
MAIN TOPICS OF STUDY
Demonstration of use of volume and weight measurements devices.
Titration of weak acid-weak base.
Quantitative Test for carbohydrates
Distinguish reducing and nontraducing sugars.
Using ninhydrin for distinguishing Imino and amino acids
Protein estimation by Biuret and Lowry’s methods.
Protein estimation by Bradfrd colorometric methods.
Extraction of lipids and analysis by TLC.
Estimation of nucleus ends by absorbance at 260mm and hyper chromicity.
Enzymatic assay of phosphates.
Hydrolysis of starch by an enzyme
TOTAL: 60 PERIODS
REFERENCES
1. Wilson and Walker “Principles and Techniques of Practical Biochemistry” 4 Edn.,
Cambridge Knew pros 1997.
2. Plummer DT “An Introduction to Practical Biochemistry” III Edn., Tata McGrawhill.
LIST OF EQUIPMENTS
Heating Mantles (5) / Water Baths (5) / Bunsen Buners (10)
TLC Plates – Required Numbers
Colorimeter – 2 Nos.
Consumables and Reagents.
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