BT2302 BIOCHEMISTRY-II SYLLABUS | ANNA UNIVERSITY B.TECH. BIOTECHNOLOGY 5TH SEMESTER SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FIFTH SEMESTER B.TECH. BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS, IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI,TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009
BT2302 BIOCHEMISTRY-II L T P C
3 0 0 3
AIM
To develop skills of the students in Biochemistry with special emphasis on the
metabolizing amino acids, nucleic acids, polysaccharide & lipids and an bio membranes.
This may be a pre-requisite for certain-elective courses like Metabolic Engineering;
Molecular Modelling & Drug Design etc.
OBJECTIVES
At the end of the course, the student would have gained an extensive knowledge of
Biochemistry particular various metabolic pathways & Biomembranes. This
knowledge will be useful for project work.
UNIT I METABOLISM OF AMINO ACIDS 15
Nitrogen metabolism and urea cycle. Biosynthesis of Gly, Ser and Cys; Biosynthesis of
six essential amino acids (Met, Thr, Lys, Ile, Val, Leu) and regulation of branched chain
amino acids (concerted inhibition, allosteric regulation and enzyme multiplicity,
sequential feed back) from oxaloacetate and pyruvate; Biosynthesis of aromatic amino
acids. Metabolic disorders associated with branched chain and aromatic amino acid
degradation. Important molecules derived from amino acids (auxins, DOPA, Serotonin,
porphyrins, T3, T4, Adrenaline, Noradrenaline, histamine, GABA, polyamines etc)
UNIT II PROTEIN TRANSPORT AND DEGRADATION 5
Protein targeting, signal sequence, secretion; Folding, Chaperons and targeting of
organelle proteins, Protein degradation, receptor-mediated endocytosis, turnover.
UNIT III METABOLISM OF NUCLEIC ACIDS, POLYSACCHARIDES AND
LIPIDS 10
Biosynthesis of nucleotides, denovo and salvage pathways for purines and pyrimidines,
regulatory mechanisms: Degradation of nucleic acid by exo and endo nucleases.
Biosynthesis and degradation of starch and glycogen, Biosynthesis and degradation of
Lipids: Fatty acid synthesis and oxidative degradation, Triacylglycerol and phospholipid
biosynthesis and degradation; Cholesterol biosynthesis and regulation and targets and
action of cholesterol lowering drugs. Vitamins (fat and water-soluble), Co-enzymes,
hormones (steroids like corticoids, amino acids derived like adrenaline and
noradrenaline and peptides like insulin and growth hormone).
UNIT IV STRUCTURAL PROTEINS AND CYTOSKELETON 5
Contractile proteins, Actin, myosin, actin polymerization, acto-myosin complexes,
mechanism of myosin ATPase activity, excitation- contraction coupling and relaxation,
microtubules, microfilaments and their role in organelle movements
UNIT V BIOMEMBRANE, TRANSPORT AND ELECTRICAL
CONDUCTIVITY 10
Micelles, lipid bi-layer structure of membranes, membrane proteins, passive, careermediated
and active transport, ion-selective channels, trans-membrane potential
coupled ATP generation, receptors, acetylcholine receptor as a ligand gated ionchannel,
Neuronal sodium channel as voltage-gated ion channel, neurotransmitters and
39
their mechanism of action, action potential, depolarization and nerve conduction. Ionchannel
agonists and antagonists as drugs. Ion channel defects (Cystic Fibrosis)
TOTAL : 45 PERIODS
TEXT BOOKS
1. Lehninger's Principles of Biochemistry by David L. Nelson and Michael M Cox,
Macmillan Worth Publisher
2. Lubert Stryer, Biochemistry, 4th Edition, WH Freeman & Co., 2000.
REFERENCES
1. Voet and Voet, Biochemistry, 2nd Edition, John Wiley & Sons Inc., 1995.
2. Murray, R.K., Granner, B.K., Mayes, P.A., Rodwell. V.W., Harper’s Biochemistry,
Prentice Hall International.
3. Creighton. T.E., Proteins, Structure and Molecular Properties, 2nd Edition, W.H.
Freeman and Co., 1993.
4. Salway, J.G., Metabolism at a Glance, 2nd Edition, Blackwell Science Ltd., 2000.
BT2302 BIOCHEMISTRY-II L T P C
3 0 0 3
AIM
To develop skills of the students in Biochemistry with special emphasis on the
metabolizing amino acids, nucleic acids, polysaccharide & lipids and an bio membranes.
This may be a pre-requisite for certain-elective courses like Metabolic Engineering;
Molecular Modelling & Drug Design etc.
OBJECTIVES
At the end of the course, the student would have gained an extensive knowledge of
Biochemistry particular various metabolic pathways & Biomembranes. This
knowledge will be useful for project work.
UNIT I METABOLISM OF AMINO ACIDS 15
Nitrogen metabolism and urea cycle. Biosynthesis of Gly, Ser and Cys; Biosynthesis of
six essential amino acids (Met, Thr, Lys, Ile, Val, Leu) and regulation of branched chain
amino acids (concerted inhibition, allosteric regulation and enzyme multiplicity,
sequential feed back) from oxaloacetate and pyruvate; Biosynthesis of aromatic amino
acids. Metabolic disorders associated with branched chain and aromatic amino acid
degradation. Important molecules derived from amino acids (auxins, DOPA, Serotonin,
porphyrins, T3, T4, Adrenaline, Noradrenaline, histamine, GABA, polyamines etc)
UNIT II PROTEIN TRANSPORT AND DEGRADATION 5
Protein targeting, signal sequence, secretion; Folding, Chaperons and targeting of
organelle proteins, Protein degradation, receptor-mediated endocytosis, turnover.
UNIT III METABOLISM OF NUCLEIC ACIDS, POLYSACCHARIDES AND
LIPIDS 10
Biosynthesis of nucleotides, denovo and salvage pathways for purines and pyrimidines,
regulatory mechanisms: Degradation of nucleic acid by exo and endo nucleases.
Biosynthesis and degradation of starch and glycogen, Biosynthesis and degradation of
Lipids: Fatty acid synthesis and oxidative degradation, Triacylglycerol and phospholipid
biosynthesis and degradation; Cholesterol biosynthesis and regulation and targets and
action of cholesterol lowering drugs. Vitamins (fat and water-soluble), Co-enzymes,
hormones (steroids like corticoids, amino acids derived like adrenaline and
noradrenaline and peptides like insulin and growth hormone).
UNIT IV STRUCTURAL PROTEINS AND CYTOSKELETON 5
Contractile proteins, Actin, myosin, actin polymerization, acto-myosin complexes,
mechanism of myosin ATPase activity, excitation- contraction coupling and relaxation,
microtubules, microfilaments and their role in organelle movements
UNIT V BIOMEMBRANE, TRANSPORT AND ELECTRICAL
CONDUCTIVITY 10
Micelles, lipid bi-layer structure of membranes, membrane proteins, passive, careermediated
and active transport, ion-selective channels, trans-membrane potential
coupled ATP generation, receptors, acetylcholine receptor as a ligand gated ionchannel,
Neuronal sodium channel as voltage-gated ion channel, neurotransmitters and
39
their mechanism of action, action potential, depolarization and nerve conduction. Ionchannel
agonists and antagonists as drugs. Ion channel defects (Cystic Fibrosis)
TOTAL : 45 PERIODS
TEXT BOOKS
1. Lehninger's Principles of Biochemistry by David L. Nelson and Michael M Cox,
Macmillan Worth Publisher
2. Lubert Stryer, Biochemistry, 4th Edition, WH Freeman & Co., 2000.
REFERENCES
1. Voet and Voet, Biochemistry, 2nd Edition, John Wiley & Sons Inc., 1995.
2. Murray, R.K., Granner, B.K., Mayes, P.A., Rodwell. V.W., Harper’s Biochemistry,
Prentice Hall International.
3. Creighton. T.E., Proteins, Structure and Molecular Properties, 2nd Edition, W.H.
Freeman and Co., 1993.
4. Salway, J.G., Metabolism at a Glance, 2nd Edition, Blackwell Science Ltd., 2000.
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