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Monday, September 10, 2012

EN3218 MICROBIOLOGY LAB SYLLABUS | ANNA UNIVERSITY BE ENVIRONMENTAL ENGINEERING 4TH SEM SYLLABUS REGULATION 2008 2011 2012-2013

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EN3218 MICROBIOLOGY LAB SYLLABUS | ANNA UNIVERSITY BE ENVIRONMENTAL ENGINEERING 4TH SEM SYLLABUS REGULATION 2008 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FOURTH SEMESTER BE ENVIRONMENTAL ENGINEERING DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI,TIRUNELVELI,COIMBATORE), 2008 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009


EN3218 MICROBIOLOGY LABORATORY L T P C
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OBJECTIVES
To understand the experimental procedures involved in the study of micro-organisms
and the method of testing their presence.
1. Sampling Techniques
2. Sterilization Techniques
3. Preparation of culture media
4. Isolation of micro-organisms (Air, water, soil, sediment)
5. Isolation of anaerobic sediments (Sewage sediments)
6. Isolation of yeast (Sediment)
7. Purification of micro-organisms
8. Cultural characteristics of bacteria
9. Simple staining
10. Negative staining
11. Differential staining
12. Acid-fast staining
13. Coliform test
14. MPN test
TOTAL: 45 PERIODS
LIST OF EQUIPMENTS
(For a batch of 30 students)
1. Incubator - 1 No.
2. Incubator Shaker - 3 Nos.
3. Microscopes - 5 Nos.
4. Micropipettes - 3 in each volume
5. Colony counter - 1 No.
6. Anaerobic jar - 2 Nos.
7. Laminar hood - 1 No.
8. Chemicals - 1 lot
9. Petty dishes - 5 Nos.
10. Innoculum loops - 5 Nos.
11. Glass slides - 5 Nos.
12. China dish - 10 Nos.
13. Staining Kit - 5 Nos.
14. Refrigerator - 1 No.

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