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Saturday, November 3, 2012

BT9211 BIOCHEMICAL ENGINEERING AND FERMENTATION TECHNOLOGY SYLLABUS | ANNA UNIVERSITY MTECH BIOTECHNOLOGY 1ST SEM SYLLABUS REGULATION 2009 2011 2012-2013

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BT9211 BIOCHEMICAL ENGINEERING AND FERMENTATION TECHNOLOGY SYLLABUS | ANNA UNIVERSITY MTECH BIOTECHNOLOGY 1ST SEM SYLLABUS REGULATION 2009 2011 2012-2013 BELOW IS THE ANNA UNIVERSITY FIRST SEMESTER M.TECH BIOTECHNOLOGY DEPARTMENT SYLLABUS, TEXTBOOKS, REFERENCE BOOKS,EXAM PORTIONS,QUESTION BANK,PREVIOUS YEAR QUESTION PAPERS,MODEL QUESTION PAPERS, CLASS NOTES, IMPORTANT 2 MARKS, 8 MARKS, 16 MARKS TOPICS. IT IS APPLICABLE FOR ALL STUDENTS ADMITTED IN THE YEAR 2011 2012-2013 (ANNA UNIVERSITY CHENNAI,TRICHY,MADURAI, TIRUNELVELI,COIMBATORE), 2009 REGULATION OF ANNA UNIVERSITY CHENNAI AND STUDENTS ADMITTED IN ANNA UNIVERSITY CHENNAI DURING 2009

BT9211 BIOCHEMICAL ENGINEERING AND FERMENTATION TECHNOLOGY L T P C
3 0 0 3
UNIT I INTRODUCTION TO BIOPROCESSES: 5
Historical development of bioprocess technology, An overview of traditional and modern
applications of biotechnological processes, general requirements of fermentation
processes, Basic design and construction of fermentor and ancillaries, Main parameters
to be monitored and controlled in fermentation processes.
UNIT II METABOLIC STOICHIOMETRY AND ENERGETICS 8
Stoichiometry of Cell growth and product formation, elemental balances, degrees of
reduction of substrate and biomass, available electron balances, yield coefficients of
biomass and product formation, maintenance coefficients Energetic analysis of microbial
growth and product formation, oxygen consumption and heat evolution in aerobic
cultures, thermodynamic efficiency of growth.
UNIT III MEDIA DESIGN FOR FERMENTATION PROCESSES 12
Medium requirements for fermentation processes, Carbon, nitrogen, minerals, vitamins
and other complex nutrients, oxygen requirements, medium formulation of optimal
growth and product formation, examples of simple and complex media. Medium for plant
cell culture and animal cell culture. Medium design of commercial media for industrial
fermentations – Plackett burman design, response surface methodology, simplex
design, continuous cultivation method to determine the kinetic parameters and
maintenance coefficient and pulse &shift method of medium optimiuzation. Case studies
on each medium design methods.
UNIT IV KINETICS OF MICROBIAL GROWTH AND PRODUCT FORMATION
10
Phases of cell growth in batch cultures, Fed batch and continuous cultures. Simple
unstructured kinetic models for microbial growth, Monod model, Growth of filamentous
organisms & yeast. Growth associated (primary) and non-growth associated (secondary)
product formation kinetics, Leudeking-Piret models, substrate and product inhibition on
cell growth and product formation.
UNIT V FERMENTATION TECHNOLOGY 10
Case studies on production of Lactic acid, Glutamic acid, Pencillin, Microbial Lipase and
Protease, Recombinant Insulin. Case studies should deal with strain improvement,
medium designs, process optimization etc.,
TOTAL : 45 PERIODS
REFERENCES
1. Bailey, J.E. and Ollis, D.F. Biochemical Engineering Fundamentals", 2nd
ed.,McGraw Hill 1986.
2. Shuler, M.L. and Kargi, F. Bioprocess Engineering : Basic concepts, 2nd ed.,
Prentice-Hall, 2002.
3. Doran Pauline M, Bioprocess Engineering Principles, Academic Press, 1995
4. Stanbury, P.F., Stephen J. Hall & A. Whitaker, Principles of Fermentation
Technology, Science & Technology Books.

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